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Angiotensin-I converting enzyme (ACE) inhibitory tripeptides from rice protein hydrolysate: Purification and characterization

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成果类型:
期刊论文
作者:
Chen, Jiwang*;Liu, Shanshan;Ye, Ran;Cai, Guangxia;Ji, Bo;...
通讯作者:
Chen, Jiwang
作者机构:
[Cai, Guangxia; Wu, Yongning; Liu, Shanshan; Chen, Jiwang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
[Ye, Ran] Univ Tennessee, Dept Biosyst Engn & Soil Sci, Knoxville, TN 37996 USA.
[Ji, Bo] Univ Tennessee, Dept Anim Sci, Knoxville, TN 37996 USA.
[Wu, Yongning] Chinese Ctr Dis Control & Prevent, Inst Nutr & Food Safety, Beijing 100021, Peoples R China.
通讯机构:
[Chen, Jiwang] W
Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
语种:
英文
关键词:
ACE-inhibitory peptides;Antihypertensive effect;Inhibition pattern;Purification;Rice protein hydrolysate;Stability against gastrointestinal proteases
期刊:
Journal of Functional Foods
ISSN:
1756-4646
年:
2013
卷:
5
期:
4
页码:
1684-1692
基金类别:
state scholarship fund for China; National Advanced Science and Technology (863) Key ProgramNational High Technology Research and Development Program of China [2010AA023003]; Young and Middle-aged Talent Project in Hubei Province [Q20091808]
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
摘要:
In this study, angiotensin-I converting enzyme (ACE) inhibitory tripeptides from rice protein hydrolysate were purified and their properties were further characterized. Val-Asn-Pro (VNP) and Val-Trp-Pro (VWP) were successfully purified from rice protein hydrolysate through multi-step isolation and purification comprising of ultrafiltration and DA201-C macroporous adsorption resin, followed by a two-step reverse phase high performance liquid chromatography (RP-HPLC). The sequences of tripeptides were separately assayed by using automated protein/peptide sequencer. In addition, the stability of ...

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