Multiple effects driven by pulsed electric field to enhance the catalytic efficiency of the mussel-inspired proteolytic membrane in protein hydrolysis

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成果类型：

期刊论文

作者：

Chen, Zhe;Zhu, Shiyu;Zhang, Haoran;Wang, Fang;Marsza, Krystian;...

通讯作者：

Zhu, ZZ;Wang, F

作者机构：

[Zhu, Zhenzhou; Zhang, Haoran; Chen, Zhe; Zhu, Shiyu] Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.

[Marsza, Krystian; Chen, Zhe] Prof Waclaw Dabrowski Inst Agr & Food Biotechnol, State Res Inst, Dept Fruit & Vegetable Prod Technol, 36 Rakowiecka St, PL-02532 Warsaw, Poland.

[Marsza, Krystian] Univ Rzeszow, Inst Food Technol & Nutr, Coll Nat Sci, Dept Food Technol & Human Nutr, Zelwerowicza 2D, PL-35601 Rzeszow, Poland.

[Wang, F; Wang, Fang] Wuhan Inst Technol, Sch Chem Engn & Pharm, Key Lab Green Chem Proc, Minist Educ,Key Lab Novel Reactor & Green Chem Tec, Wuhan 430205, Peoples R China.

通讯机构：

[Zhu, ZZ ; Wang, F ] W

Wuhan Polytech Univ, Sch Modern Ind Selenium Sci & Engn, Wuhan 430023, Peoples R China.

Wuhan Inst Technol, Sch Chem Engn & Pharm, Key Lab Green Chem Proc, Minist Educ,Key Lab Novel Reactor & Green Chem Tec, Wuhan 430205, Peoples R China.

语种：

英文

关键词：

Charge interaction;PDA and PEI co-deposition;Proteolytic membrane;Pulsed electric field

期刊：

Chemical Engineering Journal

ISSN：

1385-8947

年：

2023

卷：

453

页码：

139792

DOI：

10.1016/j.cej.2022.139792

基金类别：

The authors acknowledge the support of outstanding young and middle-aged science and technology innovation team in Hubei Province ( T2020012 ), Key Research and Development Program of Hubei Province ( 2020BBA043 ), Scientific and Technology Project of Enshi Tujia & Miao Autonomous Prefecture ( XYJ2020000021 ) and Key Innovation Team in Enshi Tujia and Miao Autonomous Prefecture, 2020 .

机构署名：

本校为第一且通讯机构

院系归属：

硒科学与工程现代产业学院

摘要：

Although the immobilization of enzymes on the membrane is an effective method to improve enzyme stability and reusability, the immobilized enzyme also causes low activity and reduces substrate accessibility. In order to overcome these shortcomings and provide a viable proteolytic membrane with increasing enzyme activity and substrate accessibility, a pulsed electric field (PEF)-assisted proteolytic membrane (PAPM) was constructed by immobilizing PEF-treated pepsin on PDA and PEI co-deposited ultrafiltration membrane. The PEF-treated pepsin endo...

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Multiple effects driven by pulsed electric field to enhance the catalytic efficiency of the mussel-inspired proteolytic membrane in protein hydrolysis

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