期刊论文

Xiao, Xianjin;Xiong, CL

英文

中国化学快报：英文版

1001-8417

2023

34

2

338-342

10.1016/j.cclet.2022.04.016

This work was supported by the National Natural Science Foundation of China (Nos. 21705053 and 81871732), the Open Foundation of NHC Key Laboratory of Birth Defects Prevention (No. ZD202104) and the Open Foundation of Key Laboratory of Birth Regulation and Control Technology of National Health Commission of China, Maternal Child Health Care Hospital of Shandong Province. We were grateful for the single-cell analysis system provided by Jiangsu Rayme Biotechnology Co., Ltd. and their technical support for the construction of SSRDM in this work.

本校为其他机构

Sperm damage caused by reactive oxygen species (ROS) is one of the main causes of male infertility. Therefore, the level of ROS in sperm is an important indicator for the diagnosis and prognosis of male infertility. Herein, we constructed a single sperm ROS detection method (SSRDM) with an optical micro-probe fabricated via focused ion beam process. The micro-probe is used to separately excite fluorescence in the sperm and the area around the sperm after ROS staining, and the difference in fluorescence val-ues can reflect the level of ROS in the sperm. We collected 102 semen samples and 72 of ...