期刊论文

The differentiation of cortical and mesencephalic neural stem cells into TH positive cells in vitro induced by ascorobic acid

中文

解剖学研究

1671-0770

2009

31

2

119-121+162

基金项目：武汉工业学院校立青年重点资助基金2006Q11

本校为第一机构

目的 探讨鼠胚脑皮质区与中脑区来源的神经干细胞向酪氨酸羟化酶(tyrosine hydmxylase,TH)阳性细胞分化的差异.方法 体外分别培养小鼠胚脑皮质区与中脑区来源的神经干细胞,传代并分组,以抗坏血酸(ascorbic acid,AA)作诱导剂,在有血清条件下分别予以10 μmol、100 μmol、200 μmol AA诱导分化,并设空白组对照,DAPI标记细胞核,分化结果行免疫荧光细胞化学技术鉴定,并镜下计数阳性细胞比例.结果 经诱导,中脑神经干细胞在AA作用下各组均可分化出TH阳性细胞,其中比例最高约9%;皮质神经干细胞各组均未见分化出TH阳性细胞.结论 在本实验条件下,中脑神经干细胞在AA作用下可以明显分化出TH阳性细胞,而皮...

Objective To compare cortical neural stem cells （C-NSCs） with mesencephalic neural stem cells （M-NSCs） on the capabilities of differentiation into tyrosine hydroxylase positive （TH-ir） cells in vitro. Methods The two kinds of NSCs were cultured and passaged in vitro, then three doses of ascrobic acid （AA）, including 10,100 and 200 p~mol AA, were respectively used to induce them to differentiate in the condition of DMEM/F12 medium with 10% FBS. Immunocytochemical techniques and DAPI-cell-count assessment were used to detect the TH-ir cel...