Effect of pulsed electric field on assembly structure of α-amylase and pectin electrostatic complexes

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

Jin, Weiping*;Wang, Zhifeng;Peng, Dengfeng;Shen, Wangyang;Zhu, Zhenzhou;...

通讯作者：

Jin, Weiping

作者机构：

[Cheng, Shuiyuan; Shen, Wangyang; Zhu, Zhenzhou; Jin, Weiping; Huang, Qingrong; Wang, Zhifeng] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.

[Cheng, Shuiyuan; Shen, Wangyang; Zhu, Zhenzhou; Jin, Weiping; Huang, Qingrong; Wang, Zhifeng] Wuhan Polytech Univ, Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

[Huang, Qingrong] Rutgers State Univ, Food Sci, 65 Dudley Rd, New Brunswick, NJ 08901 USA.

[Li, Bin; Peng, Dengfeng] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China.

通讯机构：

[Jin, Weiping] W

Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.

语种：

英文

关键词：

Assembling behavior;Electrostatic interaction;Particles motion;Protein/Polysaccharide complex;Pulsed electric field

期刊：

Food Hydrocolloids

ISSN：

0268-005X

年：

2020

卷：

101

页码：

105547

DOI：

10.1016/j.foodhyd.2019.105547

基金类别：

Author Contribution Weiping Jin: Conceptualization, Methodology, Writing original draft, acquisition Zhifeng Wang: Data curation, Formal analysis Dengfeng Peng: Software, Writing review & editing Wangyang Shen: Resources Zhenzhou Zhu: Methodology Shuiyuan Cheng: Project administration Bin Li: Supervision Qingrong Huang: Visualization, Writing review & editing.

机构署名：

本校为第一且通讯机构

院系归属：

食品科学与工程学院

摘要：

Pulsed electric field (PEF) could change the charge distribution of proteins and polysaccharides and affect their interactions and complexes aggregation, but those influences are not enough evaluated. Here, the effects of PEF on the complexes of alpha-amylase and pectin driven by electrostatic binding were studied. Changes in molecular conformation of alpha-amylase and assembly structure of alpha-amylase/pectin complexes were orderly assessed by fluorescence, FTIR, DSC, enzyme activities, particle size, zeta-potential, CLSM, and SEM. After PEF treatment (E similar to 20 kV/cm, t(exp)similar to...

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Effect of pulsed electric field on assembly structure of α-amylase and pectin electrostatic complexes

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