期刊论文

Molecular Modification of Lipase from Rhizopus oryzae and Its Application in Waste Oil Conversion

中文

生物化工

2096-0387

2022

8

3

9-12,35

10.3969/j.issn.2096-0387.2022.03.003

湖北省重点研究计划项目(2021BCA113)。

本校为第一机构

利用液态脂肪酶将废弃油脂转换为清洁能源生物柴油，对环境保护以及工业生产具有实际意义与价值。通过分子改造米根霉脂肪酶（ROL），将61位上的丝氨酸突变成苏氨酸（S61T），将150位点上的谷氨酸胺突变为亮氨酸（G150L），提高其在毕赤酵母X-33中的表达，并利用高效的液态脂肪酶将废弃油脂转化为生物柴油。改造后的米根霉表达菌株在摇瓶条件下发酵酶活最高达到1 430 U/mL，酶活提高了5.1倍。

Using liquid lipase to convert waste oil into clean energy biodiesel has practical significance and value for environmental protection and industrial production.The expression of Rhizopus oryzae lipase(ROL)in Pichia pastoris X-33 was improved by molecular modification such as changed the Ser 61 into Thr(S61T),and changed Glu 150 into Leu(G150L).The waste oil was converted into biodiesel by efficient lipase.After transformation,the highest enzyme activity of ROL expression strain reached 1430 U/mL...