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Detection of type I collagen fibrils formation and dissociation by a fluorescence method based on thioflavin T

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成果类型:
期刊论文
作者:
Zou, Meilian;Yang, Huan;Wang, Haibo*;Wang, Haiyin;Zhang, Juntao*;...
通讯作者:
Wang, Haibo;Zhang, Juntao
作者机构:
[Zhang, Hanjun; Yang, Huan; Zou, Meilian] Wuhan Polytech Univ, Sch Food Sci & Engn, Wuhan, Hubei, Peoples R China.
[Xie, Dong; Wei, Benmei; Wang, Haibo; Zhang, Juntao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.
[Wang, Haiyin] Huzhong Univ Sci & Technol, Tongji Med Coll, Wuhan, Hubei, Peoples R China.
通讯机构:
[Wang, HB; Zhang, JT] W
Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan, Hubei, Peoples R China.
语种:
英文
关键词:
Collagen;Fibrillogenesis;Thioflavin T
期刊:
International Journal of Biological Macromolecules
ISSN:
0141-8130
年:
2016
卷:
92
页码:
1175-1182
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21376183]; Project of Application and Foundation Study of Wuhan, China [2013020501010177]; Innovation Team Program of Hubei province, China [T201208]; Research and Innovation Initiatives of WHPU, China [2016J04]
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
化学与环境工程学院
摘要:
In this study, fibrillogenesis and thermal dissociation of pepsin-soluble collagen (PSC), extracted from snakehead (Channa argus) skin, were monitored by fluorescence method based on thioflavin T (Th-T), where the accuracy and sensitivity were evaluated and compared with those of turbidity assay. The fluorescence method revealed the fibrillogenesis dynamics of collagen with better sensitivity, especially at nucleation and plateau stages. The melting temperature (T-m) of PSC was estimated to be 47 degrees C by circular dichroism spectroscopy; below this temperature, the triple-helical structure...

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