The preparation conditions of dietary fiber was studied from such points of view as the raw material, extracting method and bleaching. The results showed that the fresh wheat bran got higher extracting rate of dietary fiber than the stale wheat bran; higher consistency of compound amylase and sodium hydroxide resulted in lower extracting rate and higher purity of dietary fiber; enzyme method obtained higher extracting rate and lower purity of dietary fiber than alkali method; the bleaching effect of the mixture of hydrogen peroxide and chlorine water was superior to that of individually used h...