Production of the antidepressant orcinol glucoside in Yarrowia lipolytica with yields over 6,400-fold higher than plant extraction

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成果类型：

期刊论文

作者：

Chen, Bihuan;Liu, Xiaonan;Wang, Yina;Bai, Jie;Liu, Xiangyu;...

通讯作者：

Zi, Shuhui;Yang, SC;Jiang, HF

作者机构：

[Yang, Shengchao; Xiang, Guisheng; Liu, Xiangyu; Zi, Shuhui; Zhang, Guanghui; Wang, Yina; Yang, SC; Chen, Bihuan] Yunnan Agr Univ, Coll Agron & Biotechnol, Natl & Local Joint Engn Res Ctr Germplasm Innovat, Key Lab Med Plant Biol Yunnan Prov, Kunming, Yunnan, Peoples R China.

[Lu, Lina; Liu, Wei; Jiang, Huifeng; Liu, Xiaonan; Zhu, Xiaoxi; Cheng, Jian; Chen, Bihuan; Jiang, HF; Bai, Jie] Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Engn Biol Low Carbon Mfg, Tianjin, Peoples R China.

[Dai, Zongjie; Lu, Lina; Zhang, Ge; Liu, Wei; Jiang, Huifeng; Liu, Xiaonan; Zhu, Xiaoxi; Cheng, Jian; Chen, Bihuan; Jiang, HF; Bai, Jie] Natl Ctr Technol Innovat Synthet Biol, Tianjin, Peoples R China.

[Yang, Shengchao; Xiang, Guisheng; Liu, Xiangyu; Zi, Shuhui; Zhang, Guanghui; Wang, Yina; Yang, SC; Chen, Bihuan] Yunnan Characterist Plant Extract Lab, Kunming, Yunnan, Peoples R China.

[Liu, Xiaonan; Zhu, Xiaoxi] Univ Chinese Acad Sci, Beijing, Peoples R China.

通讯机构：

[Yang, SC ; Zi, SH] Y

[Jiang, HF ] C

Yunnan Agr Univ, Coll Agron & Biotechnol, Natl & Local Joint Engn Res Ctr Germplasm Innovat, Key Lab Med Plant Biol Yunnan Prov, Kunming, Yunnan, Peoples R China.

Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Engn Biol Low Carbon Mfg, Tianjin, Peoples R China.

Natl Ctr Technol Innovat Synthet Biol, Tianjin, Peoples R China.

语种：

英文

关键词：

Fermentation;Biosynthesis;Plasmid construction;Transcriptome analysis;Bioengineering;Glucose;Phylogenetic analysis;Antidepressants

期刊：

PLOS Biology

ISSN：

1544-9173

年：

2023

卷：

期：

页码：

e3002131

DOI：

10.1371/journal.pbio.3002131

基金类别：

Major Science and Technology Projects of Yunnan Province [202102AA310048-1]; Science and Technology Innovation Team of Yunnan [202105AE160011]; Independent Research Fund of Yunnan Characteristic Plant Extraction Laboratory [2022YKZY001]; Major Science and Technology Programs in Yunnan Province [2018ZF011, 202102AA310045]; Tianjin Science Fund for Distinguished Young Scholars [18JCJQJC48300]; Science and Technology Partnership Program, Ministry of Science and Technology of China [KY202001017]; COMSATS Joint Center for Industrial Biotechnology [TSBICIP-IJCP-001-01]; National Key R&D Program of China [2021YFC2103500]

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本校为其他机构

院系归属：

生命科学与技术学院

摘要：

Orcinol glucoside (OG), mainly found in the rhizome of the traditional Chinese herb Curculigo orchioides Gaertn, is noted for its antidepressant effects. In this study, an efficient screening pipeline was established for identifying the highly active orcinol synthase (ORS) and UDP-dependent glycosyltransferase (UGT) involved in the biosynthesis of OG by combining transcriptome analysis, structure-based virtual screening, and in vitro enzyme activity assays. By enhancing the downstream pathway, metabolic engineering and fermentation optimization, the OG production in Yarrowia lipolytica was imp...

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Production of the antidepressant orcinol glucoside in Yarrowia lipolytica with yields over 6,400-fold higher than plant extraction

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