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Knockout of Noxa with CRISPR/Cas9 Increases Host Resistance to Influenza Virus Infection

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成果类型:
期刊论文
作者:
Zhou, Ao;Zhang, Wenhua;Wang, Baoxin;Dong, Xia;Zhang, Jing;...
通讯作者:
Chen, H.
作者机构:
[Wang, Baoxin; Zhang, Jing; Dong, Xia; Zhou, Ao; Zhang, Wenhua; Chen, Hongbo] Wuhan Polytech Univ, Sch Anim Sci & Nutr Engn, Lab Genet Breeding Reprod & Precis Livestock Farmi, Wuhan 430023, Hubei, Peoples R China.
[Wang, Baoxin; Zhang, Jing; Dong, Xia; Zhou, Ao; Zhang, Wenhua; Chen, Hongbo] Hubei Prov Ctr Technol Innovat Domest Anim Breedin, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Chen, H.] L
Laboratory of Genetic Breeding Reproduction and Precision Livestock Farming, Hubei, China
语种:
英文
关键词:
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
期刊:
Cellular Microbiology
ISSN:
1462-5814
年:
2023
卷:
2023
基金类别:
National Natural Science Foundation of China [81902073]; Hubei Key Laboratory of Animal Embryo and Molecular Breeding [KLAEMB-2021-01]; Wuhan Polytechnic University
机构署名:
本校为第一机构
院系归属:
动物科学与营养工程学院
摘要:
The influenza virus induces cellular apoptosis during viral propagation, and controlling this virus-induced apoptosis process has been shown to have significant antiviral effects. The proapoptotic BH3-only protein Noxa is a strong inducer of apoptosis that can be activated by this virus, suggesting that Noxa has the potential as an anti-influenza target. To assess the value of Noxa as an antiviral target, we utilized CRISPR/Cas9 technology to produce a Noxa-knockout cell line. We found that the knockout of Noxa resulted in a dramatic reduction in the cytopathic effect induced by the influenza ...

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