期刊论文

中文

浙江大学学报(农业与生命科学版)

1008-9209

2008

34

2

175-180

10.3785/j.issn.1008-9209.2008.02.009

湖北省自然科学基金资助项目（2005ABA092）； 武汉工业学院创新团队基金资助项目；

本校为第一机构

研究制备能有效用于金霉素残留检测的特异性多克隆抗体．采用戊二醛法和混合酸酐法合成2种不同的金霉素-BSA完全抗原，免疫新西兰大白兔制备多克隆抗体，应用ELISA法鉴定抗金霉素多克隆抗体．结果表明，2种血清的抗体效价最高均达3．2×10~5，而戊二醛法制备的抗原产生的抗体具有更高的亲和力，抑制率为50％时的金霉素的质量浓度（IC50）为96．2μg·mL~(-1)，金霉素的最低检测限为6．67ng·mL~(-1)，金霉素和四环素的交叉反应率为77％．研究结果为畜产品中四环素类抗生素残留的检测奠定了较好的基础．

Anti chlortetracycline（CTC）polyclonal antibodies for effective residue detection were produced and studied. Two kinds of CTC immunogens were prepared via glutaraldehyde and mixed acid anhydride methods. New Zealand rabbits were immunized with these immunogens to produce the polyclonal antibodies, which were identified by indirect enzyme-linked immunosorbent assay （ELISA）. Results indicate that the serum titers of the two kinds of antibodies all reached 3.2 × 10^5 , however, the antibody produced by the immunogen prepared via glutaraldehyde...