期刊论文

Lihong Miao

英文

Protein Expression and Purification

1046-5928

2022

200

106165

10.1016/j.pep.2022.106165

Author contribution Pulin Liu: conceptualization, investigation, methodology, writing; Jingxiao Guo: methodology investigation, data curation; Hanyan Liu: investigation, data curation; Lihong Miao: conceptualization, funding acquisition, project administration. This work was supported by the National key research and development program (Grant No. 2021YFC2100202).

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Feruloyl esterase is a subclass of α/β hydrolase, which could release ferulic acid from biomass residues for use as an efficient additive in food or pharmaceutical industries. In the present study, a feruloyl esterase with broad substrate specificity was characterised and secreted by Bacillus subtilis WB600. After codon usage optimisation and signal peptide library screening, the secretion amount of feruloyl esterase was enhanced by up to 10.2-fold in comparison with the base strain. The site-specific amino acid substitutions that facilitate ...