To evaluate the contribution of GAC to the rapeseed oil degradation, 0.2 g of GAC were added into serum bottles as the experimental group. Fig. 1(a) shown the methane yield for the rapeseed oil anaerobic digestion with or without GAC. Before being transferred, methane yield of GAC-amended group and control group was almost same, but methane yield of GAC-amended group was higher than that of control groups after being transferred. At the beginning, the microorganisms were no differences, so, no