期刊论文

He, Y;Shao, YC

英文

Journal of Fungi

2309-608X

2023

9

12

1174-

10.3390/jof9121174

Y.H.: Conceptualization, Resources, Funding acquisition, Writing—review and editing, Supervision and Project administration. L.Z.: Conceptualization, Methodology, Formal analysis, Investigation, Writing—original draft preparation. X.D.: Conceptualization, Methodology, Software, and Supervision. A.L.: Methodology, Formal analysis, investigation, and Data curation; S.X.: Methodology, Formal analysis, Validation, and Software. L.W.: Methodology, Visualization, and Supervision. Y.S.: Investigation, Resources, and Supervision. All authors have read and agreed to the published version of the manuscript. This work was funded by the National Natural Science Foundation of China (No. 31701590, No. 32072188, and No. 32060526 to Y.H.), and the Outstanding Youth Program of Hubei Provincial Natural Science Foundation (No. 2023AFA102 to Y.H.).

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Citrinin (CIT), a secondary metabolite produced by the filamentous fungi Monascus species, exhibits nephrotoxic, hepatotoxic, and carcinogenic effects in mammals, remarkably restricting the utilization of Monascus-derived products. CIT synthesis is mediated through the pksCT gene and modified by multiple genetic factors. Here, the regulatory effects of two pksCT transcripts, pksCT alpha, and pksCT beta, generated via pre-mRNA alternative splicing (AS), were investigated using hairpin RNA (ihpRNA) interference, and their impact on CIT biosynthesis and the underlying mechanisms were assessed thr...