National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31301416]
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
摘要:
A high performance size exclusion-fluorescence detection (HPSEC-FD) method combined with fluorescein isothiocyanate (FITC) prelabeling was established for the microanalysis of polysaccharide–protein complexes from longan pulp (LPP). FITC-labeled LPP (LPPF) was fractionated by gel filtration chromatography. The weight-average molecular weight and FITC substitution degree of LPPF were 39.01 kDa and 0.20%, respectively. The HPSEC-FD calibration curves linear over the range of 1–200 μg/mL in mouse plasma, spleen and lung samples with correlation...