期刊论文

Optimization of Composite Enzymatic Hydrolysis Conditions of Rice Dreg Deamidization Protein

中文

粮食加工

1007-6395

2014

39

06

20-24

湖北省教育厅优秀中青年人才项目(项目编号:Q20091808)；

本校为第一机构

为了制备起泡性较高、泡沫稳定性良好的米渣发泡蛋白(rice dreg foaming protein,RDFP),采用响应面法优化了脱酰胺米渣蛋白(rice dreg deamidation protein,RDDP)的复合酶水解工艺条件。以起泡性和泡沫稳定性为指标,将碱性蛋白酶分别与中性蛋白酶、胰蛋白酶、风味蛋白酶和复合蛋白酶进行复合分步水解RDDP,确定较佳的酶组合为碱性蛋白酶和复合蛋白酶。以起泡性和泡沫稳定性综合评分为指标,通过响应面分析,建立了RDDP复合酶分步水解的动力学数学模型,模型回归相关系数为0.8951,方程显著,拟合性较好,且模型与验证试验结果吻合,可用来进行实际预测。复合酶分步水解的较佳工艺条件为:碱性蛋白酶水解时...

In this paper, the composite enzymatic hydrolysis conditions of rice dreg deamidization protein （RDDP） were optimized to prepare rice dreg foaming proteins （RDFP） with excellent foaming capacity and foam- ing stability. The combination of Alcalase and Protamex was used to hydrolyze RDDP by comparing the foaming capacity and foaming stability of the hydrolyzates to the combinations of Alcalase and other proteases including Trypsin, Neutrase, and Flavourzyme. The equation has significant and well fitting according to 0.8951 of correla- tion c...