In Vitro Packaging Mediated One-Step Targeted Cloning of Natural Product Pathway

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成果类型：

期刊论文

作者：

Tao, Weixin*;Chen, Li;Zhao, Chunhua;Wu, Jing;Yang, Dazhong;...

通讯作者：

Tao, Weixin;Sun, Yuhui

作者机构：

[Sun, Yuhui; Tao, Weixin; Deng, Zixin; Chen, Li; Tao, WX; Sun, YH] Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Hubei, Peoples R China.

[Sun, Yuhui; Tao, Weixin; Deng, Zixin; Chen, Li; Tao, WX; Sun, YH] Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Hubei, Peoples R China.

[Zhao, Chunhua] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Pharm, Wuhan 430030, Hubei, Peoples R China.

[Wu, Jing; Yang, Dazhong] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.

通讯机构：

[Tao, WX; Sun, YH] M

[Tao, WX; Sun, YH] W

Minist Educ, Key Lab Combinatorial Biosynth & Drug Discovery, Wuhan 430071, Hubei, Peoples R China.

Wuhan Univ, Sch Pharmaceut Sci, Wuhan 430071, Hubei, Peoples R China.

语种：

英文

关键词：

direct cloning;natural product;in vitro packaging;CRISPR/Cas9;gene editing

期刊：

ACS Synthetic Biology

ISSN：

2161-5063

年：

2019

卷：

期：

页码：

1991-1997

DOI：

10.1021/acssynbio.9b00248

基金类别：

National Key R&D Program of China [2018YFA0903203]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31800111]

机构署名：

本校为其他机构

院系归属：

生命科学与技术学院

摘要：

Direct cloning of natural product pathways for efficient refactoring and heterologous expression has become an important strategy for microbial natural product research and discovery, especially for those kept silent or poorly expressed in the original strains. Accordingly, the development of convenient and efficient cloning approaches is becoming increasingly necessary. Here we presented an in vitro packaging mediated cloning approach that combines CRISPR/Cas9 system with in vitro λ packaging system, for targeted cloning of natural product pa...

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In Vitro Packaging Mediated One-Step Targeted Cloning of Natural Product Pathway

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