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Activation of the NF-κ B and MAPK Signaling Pathways Contributes to the Inflammatory Responses, but Not Cell Injury, in IPEC-1 Cells Challenged with Hydrogen Peroxide

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成果类型:
期刊论文
作者:
Xiao, Kan;Liu, Congcong;Tu, Zhixiao;Xu, Qiao;Chen, Shaokui;...
通讯作者:
Liu, Yulan
作者机构:
[Liu, Congcong; Zhang, Yang; Zhang, Jing; Hu, Chien-An Andy; Liu, Yulan; Chen, Shaokui; Xiao, Kan; Xu, Qiao; Tu, Zhixiao; Wang, Xiuying] Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
[Hu, Chien-An Andy] Univ New Mexico, Dept Biochem & Mol Biol, Sch Med, Albuquerque, NM 87131 USA.
通讯机构:
[Liu, Yulan] W
Wuhan Polytech Univ, Hubei Collaborat Innovat Ctr Anim Nutr & Feed Saf, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan, Peoples R China.
语种:
英文
关键词:
Cell death;Chemical activation;Enzymes;Hydrogen peroxide;Immunology;Oxidation;Pathology;Peroxides;Epithelial cells;Inflammatory response;Jun N-terminal kinase;Lactate dehydrogenase activities;Mitogen activated protein kinase;Protein expressions;Protein translocation;Tumor necrosis factors;Cell signaling;2 (2 amino 3 methoxyphenyl)chromone;3 (4 methylphenylsulfonyl) 2 propenenitrile;4 (4 fluorophenyl) 2 (4 hydroxyphenyl) 5 (4 pyridyl)imidazole;claudin 1;hydrogen peroxide;immunoglobulin enhancer binding protein;interleukin 6;interleukin 8;lactate dehydrogenase;messenger RNA;mitogen activated protein kinase;stress activated protein kinase;tight junction protein;tumor necrosis factor;hydrogen peroxide;immunoglobulin enhancer binding protein;lactate dehydrogenase;messenger RNA;protein kinase inhibitor;stress activated protein kinase;tight junction protein;transcription factor RelA;animal cell;Article;cell damage;cell nucleus;cell viability;controlled study;enzyme activity;enzyme inhibition;enzyme phosphorylation;gene expression;inflammation;intestine injury;IPEC-1 cell line;MAPK signaling;nonhuman;protein expression;upregulation;animal;cell line;cell survival;drug effect;fluorescence;genetics;inflammation;MAPK signaling;metabolism;pathology;phosphorylation;pig;Cells;Enzymes;Hardiness;Hydrogen Peroxide;Oxidation;Pathology;Peroxides;Stresses;Animals;Cell Line;Cell Survival;Fluorescence;Hydrogen Peroxide;Inflammation;JNK Mitogen-Activated Protein Kinases;L-Lactate Dehydrogenase;MAP Kinase Signaling System;NF-kappa B;Phosphorylation;Protein Kinase Inhibitors;RNA, Messenger;Swine;Tight Junction Proteins;Transcription Factor RelA
期刊:
Oxidative Medicine and Cellular Longevity
ISSN:
1942-0900
年:
2020
卷:
2020
页码:
1-14
基金类别:
Project of Innovative Research Groups of the Natural Science Foundation of Hubei Province [2019CFA015]; Project of the Hubei Provincial Department of Education [T201508]
机构署名:
本校为第一且通讯机构
院系归属:
动物科学与营养工程学院
摘要:
Oxidative stress can lead to intestinal cell injury as well as the induction of inflammation. It is not clear whether inflammation is an important factor leading to cell injury caused by oxidative stress. The purpose of this study was to investigate the role of inflammation in intestinal injury caused by hydrogen peroxide (H2O2). Our results revealed that H2O2 stimulation significantly decreased the viability of intestinal porcine epithelial cells (IPEC-1), increased lactate dehydrogenase (LDH) activity, and disrupted the distribution of the ti...

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