期刊论文

He, Yi;He, Jingren;Chen, Fusheng

英文

Biotechnology Advances

0734-9750

2018

36

3

739-783

10.1016/j.biotechadv.2018.02.001

One of the most important technologies for heterologous gene expression is gene/pathway assembly before transformation into the heterologous host. Normally, biosynthetic PKS and NRPS gene clusters are large, ranging from 10–120 kb, and the targeted genes must be placed under the control of appropriate promoters to ensure the successful expression, which make the demands for facile DNA assembly technologies with higher efficiency, fidelity, and modularity. Various gene assembly methods can be

本校为第一且通讯机构

High throughput genome sequencing has revealed a multitude of potential secondary metabolites biosynthetic pathways that remain cryptic. Pathway reconstruction coupled with genetic engineering via heterologous expression enables discovery of novel compounds, elucidation of biosynthetic pathways, and optimization of product yields. Apart from Escherichia coli and yeast, fungi, especially Aspergillus spp., are well known and efficient heterologous hosts. This review summarizes recent advances in heterologous expression of microbial secondary metabolite biosynthesis in Aspergillus spp. We also di...