期刊论文

中文

分子细胞生物学报：英文版

1674-2788

2005

38

1

67-74

10.3321/j.issn:1673-520X.2005.01.010

教育部2002年度博士点基金（NO：20020504008）湖北省科技攻关计划项目（2003AA204B07）~~；

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分别用含10、20、40、60μmol/L的菹草类胡萝卜素提取物(CEPC)培养液处理人肝癌细胞(QGY-7703)48 h、96 h和144 h,在这三个处理时间各剂量组对肝癌细胞的抑制率平均值范围分别为0.14%-23.07%、39.59%-70.61%和71.65%-87.01%.经10、20和40μmol/L的CEPC培养液处理肝癌细胞24 h、48 h和72 h,用激光扫描共聚焦显微术(LSCM)观察细胞形态,出现了肝癌细胞数量明显减少,细胞体积缩小、皱缩变形,细胞核呈现"新月状"、条状甚至碎片状,细胞核中呈黄色的DNA面积较明显地减小等典型的凋亡细胞形态特征.以流式细胞术分析用CEPC处理肝癌细胞后各时相细胞的百分比,与对照组比较,用10μmol/L和20μmol/L浓度的CE...

Human hepatoma cell line QGY-7703 was treated with the medium containing 10, 20, 40 and 60 micromol/L carotenoids extracts from Potamogoton crispus L. (CEPC) for 48 h, 96 h and 144 h, respectively. The inhibition rates to hepatoma cells were determined by MTT assay. The results showed the average inhibition rates of the three treatments varied from 0.14%-23.07%, 39.59%-70.61% and 71.65%-87.01%, respectively. After hepatoma cells were treated with the medium containing 10, 20 and 40 micromol/L of CEPC for 24 h, 48 h and 72 h, respectively, many typical morphology features of apoptotic cells wer...