期刊论文

Yan, Da-Zhong

英文

Journal of Biotechnology

0168-1656

2017

251

166-173

10.1016/j.jbiotec.2017.04.036

This work was financially supported by the National Natural Science Foundation of China (31270112) and State Key Laboratory of Microbial Metabolism (Shanghai Jiao Tong University) (MMLKF14-06), respectively. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Author contributions Conceptualization: DZY Validation: CZL LQM DZY Formal analysis: DZY XQC Investigation: LQM CZL Resources: DZY NYZ Data curation: DZY Writing (original draft preparation): DZY XL DYL Writing (review and editing): DZY NYZ Supervision: DZY Project administration: DZY acquisition: DZY

本校为第一且通讯机构

The Gram-negative strain of Pseudomonas plecoglossicida NyZ12 isolated from soil has the ability to degrade cyclohexylamine (CHAM). The genes encoding CHAM degradation by gram-negative bacteria, however, have not been reported previously. In this study, ORFs predicted to encode CHAM degradation by NyZ12 were identified by bioinformatics analysis. Differential expression of the proposed ORFs was analyzed via RNA-seq and quantitative reverse transcription-PCR (qRT-PCR), using RNA extracted from NyZ12 cultured with or without CHAM addition. One CHAM-inducible ORF, RK21_02867 predicted to encode a...