Foundation for Innovative Research Group of Hubei Educational Office [T201619]; Youth Foundation for Science Research of Hubei Educational Office [Q20132909]; Youth Foundation for innovative research team of Huannang Normal University [201613303]
机构署名：
本校为其他机构
院系归属：
生命科学与技术学院
摘要：
Four separation methods of antimicrobial substances produced by CMN1308 (Bacillus amyloliquefaciens) were evaluated and selected according to number of antimicrobial substances and its activity in vitro. The results showed that extraction by acid precipitation of the fermentation supernatant of CMN1308 was the best with a diameter of inhibition zone of pathogen fungi P. expansum of 12.3 mm in a laboratory bioassay. Applying a silica thin layer chromatography (TLC), SDS-PAGE and other separation technologies we isolate antimicrobial substances, and the separated band were cut off for mass spect...
