期刊论文

中文

武汉大学学报(理学版)

1671-8836

2007

53

4

491-496

10.3321/j.issn:1671-8836.2007.04.021

国家高技术研究发展计划（863）项目（2004CB719606）； 农业微生物国家重点实验室开放课题经费资助；

本校为第一机构

以棉浆黑液为细菌分离培养基，从某化纤厂黑液生物处理系统中分离到16株嗜碱细菌，对其形态及生理生化等特征的研究结果表明，所分离的嗜碱细菌以产芽孢的革兰氏阴性细菌占优势，在个体形态和生理生化特性方面具有丰富的多样性。扩增rDNA限制性分析（ARDRA）结果表明，棉浆黑液中的嗜碱细菌分为2大类群，不同菌株之间的酶切图谱具有较大的差异。测定黑液生物处理系统中6株占优势的嗜碱细菌的16SrDNA序列，与国际上已报道的细菌的16SrDNA序列进行比较，结果表明：它们的序列与芽孢杆菌属（Bacillus）或副球菌属（Paracoccus）中的参比菌株的同源性达到98％以上。以16SrDNA序列为基础的Bacillus属...

Sixteen alkaliphilic strains were isolated from pulp wastewater of a fiber factory. The morphology and phenotypic characteristics showed that these trains displayed great diversity. Those strains of the gram-negative alkaliphilic bacteria producting spore were dominant. Those strains were classed into two families by amplified rDNA restriction analgsis （ARDRA） and 16S rDNA sequences, their fingerprinting patterns shows significant differences. The homology of six strains tested achieves above 98% on 16S rDNA sequences comparing to Bacillus an...