期刊论文

中文

饲料工业

1001-991X

2014

35

3

42-46

10.13302/j.cnki.fi.2014.03.011

“十二五”国家科技支撑计划[2012BAD39804] 国家973计划项目[2012CB126305] 国家自然科学基金[31372319]

本校为第一机构

研究L-谷氨酰胺（L-glutamine,GLN）,Nα-乙酰-L-谷氨酰胺（Nα-acetyl-L-glutamine,NAG）,L-精氨酸（L-arginine,ARG）,N-α-乙酰-L-精氨酸（N-alpha-acetyl-L-arginine,NAA）、瓜氨酸（L-citrulline,CIT）,α-酮戊二酸（Alpha-ketoglutaric acid,AKG）和L-脯氨酸（L-Proline,PRO）对猪小肠上皮细胞（Intestinal porcine epithelial cells,IPEC-1）细胞活力与生长的影响,比较这几种氨基酸及其衍生物对IPEC-1细胞生长的作用效果。在IPEC-1定制培养液中分别添加不同浓度的氨基酸或其衍生物培养4 d,用CCK8（cell counting kit 8）法检测细胞的活力,并用细胞计数法检测这些氨基酸或其衍生物对细胞生...

This study was conducted to investigate the effects of L-glutamine （GLN）, Nα-acetyl-L-glu- tamine （NAG）, L-arginine （ARG）, N-alpha-acetyl-L-arginine （NAA）, L-citrulline （CIT）, Alpha-keto-glularic acid （AKG） and L-Proline （PRO） on the cellular vitality and proliferation of intestinal porcine epilhelial cells （IPEC-1）. The IPEC-1 cells were incubated with the corresponding concentraliuns of amino acids or their derivatives for d days. The cellular vitality was measured by CCK8 （cell counting kil 8）, and the cell numbers were re...