期刊论文

Study on the technology of enzymatic hydrolysis of plant feed ingredients in vitro

中文

饲料工业

1001-991X

2016

37

3

49-53

10.13302/j.cnki.fi.2016.03.010

本校为第一机构

为解决直接添加植酸酶到水产饲料中不利于植酸酶充分发挥作用的问题。试验参考吉富罗非鱼饲料配方，以豆粕、棉粕和菜粕为原料，按比例为15??9??7混合，采用植酸酶进行体外水解，在单因素试验的基础上，以水解效率为指标，采用正交试验研究加酶量（A）、反应温度（B）、加水量（C）和酶解时间（D）4个不同因素对植酸酶体外水解植物性饲料原料效率的影响。结果表明，各因素对植酸酶体外水解植物性饲料原料效率的影响程度依次是A>B>C>D。其最佳酶解工艺条件为：加酶量为2000 U/kg底物，反应温度35℃，加水量50%，酶解时间为3 h。在此条件下得出的植酸酶水解效率为42.16%。

The study is to solve the problem that directly adding phytase into aquaculture feed cannot exert the action of phytase sufficiently. This experiment referenced the GIFT feed formulation ratio of 15??9??7 which is mixed of soybean meal, cottonseed meal and rapeseed meal, using hydrolysis in vi?tro of phytase, consider the hydrolysis efficiency as an indicator, apply orthogonal test based on single-factor test to research with 4 influential factors addition of enzyme amount (A), the reaction tempera?ture (B), addition of water (C) and enzymatic ...