期刊论文

中文

饲料工业

1001-991X

2016

37

6

54-58

10.13302/j.cnki.fi.2016.06.012

“十二五”国家科技支撑计划项目[2012BAD36B05]；

本校为第一机构

试验旨在研究凝结芽孢杆菌的发酵条件、发酵液的浓缩方式、载体吸附等工艺,并评价其产品性能.采用10 L全自动发酵罐发酵凝结芽孢杆菌,采用有机膜和矿物质吸附剂对发酵液进行浓缩、吸附.采用体外消化试验、体外药敏试验、耐高温试验和产品储存试验评价产品性能.结果表明:①凝结芽孢杆菌制剂的最佳工艺参数为搅拌速度200 r/min,通气量350 L/h,浓缩设备为有机陶瓷膜过滤设备,矿物质吸附剂与发酵液比例为1∶1.②产品在85℃处理10 min存活率为71％,经胃液消化后存活率为86.8％,过肠液后存活率为69％;产品在25 ℃和35℃储存30 d后仍保持50％作用的存活率,储存60 d后菌种数依旧可以到达108数量级.由此可...

This paper mainly aimed to explore optimal Bacillus coagulans fermentation conditions, con-centration process of the fermentation liquid, moreover it surveyed a evaluation on the performance ofthe product. The influence of stirring speed, throughput and constant p H for cell growth and spore wasanalysed by the production test. Concentrate and adsorb the fermented liquid to get condensate bacil-lus viable pharmaceutics. Then simulating pelleting temperature, digestion test in vitro, drug sensitivetest and storage test could be used to preliminar...