Tetrahedral DNA scaffold-based label-free electrochemical aptasensor for the detection of ochratoxin A

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成果类型：

期刊论文

作者：

Xu, Lin;Qu, Wenli;Zhu, Zhengwei;Hao, Xiaotong;Yang, Qing;...

通讯作者：

Xu, L;Li, PW

作者机构：

[Qu, Wenli; Hao, Xiaotong; Gong, Zhiyong; Xu, Lin; Liu, Liangzhong; Yang, Qing] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.

[Gong, Zhiyong; Xu, Lin; Liu, Liangzhong; Yang, Qing] Minist Educ, Key Lab Deep Proc Major Grain & Oil, Wuhan 430023, Peoples R China.

[Gong, Zhiyong; Xu, Lin; Liu, Liangzhong; Yang, Qing] Hubei Key Lab Proc & Transformat Agr Prod, Wuhan 430023, Peoples R China.

[Zhu, Zhengwei] Natl Food & Strateg Reserv Adm, Inst Mat Inspection Hubei Bur, Wuhan 430050, Peoples R China.

[Li, Peiwu; Li, PW] Chinese Acad Agr Sci, Oil Crops Res Inst, Qual Inspection & Test Ctr Oilseed Prod, Minist Agr & Rural Affairs,Natl Reference Lab Agr, Wuhan 430062, Peoples R China.

通讯机构：

[Xu, L ] W

[Li, PW ] C

Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.

Chinese Acad Agr Sci, Oil Crops Res Inst, Qual Inspection & Test Ctr Oilseed Prod, Minist Agr & Rural Affairs,Natl Reference Lab Agr, Wuhan 430062, Peoples R China.

语种：

英文

关键词：

Aptamer distribution;Sensitivity improvement;Wheat flour

期刊：

LWT

ISSN：

0023-6438

年：

2025

卷：

218

页码：

117475

DOI：

10.1016/j.lwt.2025.117475

基金类别：

National Natural Science Foundation of China [32102072]; Wuhan Knowledge Innovation Project [2022020801020399]

机构署名：

本校为第一且通讯机构

院系归属：

食品科学与工程学院

摘要：

Ochratoxin A (OTA) is a prevalently toxic mycotoxin in agro-products and foods. Therefore, the development of highly sensitive analytical methods is extremely important for OTA determination. Herein, a label-free electrochemical aptasensor based on the tetrahedral DNA nanostructure (TDN) was fabricated for the detection of OTA and compared with an aptasensor based on complementary DNA (cDNA). With the introduction of TDN, the aptamers were vertically anchored on the AuE surface resulting in reduced entanglement and non-specific adsorption. Unde...

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Tetrahedral DNA scaffold-based label-free electrochemical aptasensor for the detection of ochratoxin A

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