期刊论文

Shi, Chunlei

中文

中国食品学报

1009-7848

2015

15

8

203-210

10.16429/j.1009-7848.2015.08.029

2012AA101601:国家高技术研究发展计划（863计划） 31150001:国家自然科学基金 31471660:国家自然科学基金

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选取来自上海、宁波、舟山的109株临床、食品和环境分离株,对其进行血清分型和ERIC-PCR分型研究。通过玻片凝集反应进行血清分型,结果显示:在26种血清型中,O3∶K6和O4∶K8所占比例较高,分别为20.7%和19.5%,合计达40.2%;其次为O4∶K68,O1∶K25,O3∶K68,O3∶K25,O3∶K8和O1∶K6等。结合来源分析表明:环境分离株的血清型分布呈现出显著的多样性,与食品和临床分离株差异较大。采用ERIC-PCR基因分型方法,结合聚类分析比较各分离株之间的遗传相关性,结果显示:ERIC-PCR指纹图谱可将109株副溶血弧菌分为65个型,31个类群,分辨力指数为0.942。亲缘关系分析结果显示:不同来源的分离株可聚为一类,在基因水平...

The aim of this study was to characterize the serotype distribution and genotype polymorphism of Vibrio parahaemolyticus isolates from different sources and to provide the evidence for epidemiologic source tracking and prevention of foodborne diseases caused by V. parahaemolyticus. One hundred and nine V. parahaemolyticus isolates from patients, food and environment were typed with both serotyping and ERIC -PCR subtyping. Serotyping was conducted by slide agglutination with 11 O antibodies and 8 K antibodies. ERIC-PCR fingerprint subtyping was ...