期刊论文

Auto-immunogenicity of vimentin and megalin in murine recipients undergoing

中文

实用医学杂志

1006-5725

2015

31

10

1570-1573

10.3969/j.issn.1006-5725.2015.10.004

国家自然科学基金项目（编号:31370867）； 湖北省教育厅科研计划项目（编号:Q20121808）；

本校为第一机构

目的:研究自身组织蛋白vimentin (Vim)和megalin (Meg)在慢性移植肾肾病(CAN)大鼠体内的免疫原性.方法:Lewis大鼠接受F344大鼠供肾移植,每2周检测受体蛋白尿水平观察CAN进程.肾移植后第14、49、98天收获受体脾细胞,检测Vim及Meg特异性IFN-γ分泌细胞的数量;收获受体血清检测Vim及Meg特异性抗体水平.结果:受体肾移植6周后肾功能逐渐衰退,处于CAN病程中.Vim特异性IFN-γ分泌细胞数量逐渐增加,至98 d时其数量比对照孔差异有极显著性(126.0±10.4 vs.24.7±4.3,P＜0.001).Vim特异性抗体水平肾移植后14 d即显著升高(0.230±0.018 vs.0.063±0.008,P＜0.01),其后缓慢持续升高.CAN大鼠Meg特异性IFN-γ分...

Objective To investigate the immunogenicity of vimentin （Vim） and megalin （Meg） in murine recipients undergoing CAN. Methods The kidney of Lewis was replaced by a F344 allografi and proteinuria changes of recipients were observed every 2 weeks post-transplantation. The rats were sacrificed at 14th, 49th and 98th day after transplantation, and the spleens and serums were harvested. The frequency of specific IFN--/ secreting T ceils of Vim and Meg were detected by ELISPOT assay. The levels of Vim and Meg IgG antibodies were analyzed by ELISA....