Cloning and Expression of Apolipoprotein E3 and Its Variant apoE2 and apoE4

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成果类型：

期刊论文

论文标题(中文)：

Cloning and Expression of Apolipoprotein E3 and Its Variant apoE2 and apoE4

作者：

宗义强;刘志国;毕昊;姚研怡;过健俐;...

通讯作者：

Zong, Y.

作者机构：

[宗义强; 毕昊; 姚研怡; 过健俐; 屈伸] Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China

[刘志国] Department of Biotechnology and Chemical Engineering, Wuhan Polytechnic University, Wuhan, China

语种：

英文

关键词：

apoE2;APOE4;apoE;gene cloning;site-directed mutation;gene expression

关键词(中文)：

无性繁殖;基因表达;载脂蛋白E3;变异体

期刊：

当代医学科学

ISSN：

2096-5230

年：

2006

卷：

期：

页码：

1-3

DOI：

10.1007/BF02828023

基金类别：

This project was supported by a grant from the National Natural Sciences Foundation of China （No. 30100134）.

机构署名：

本校为其他机构

院系归属：

生命科学与技术学院

摘要：

In order to obtain three isoforms of apolipoprotein E (apoE), the cDNA encoding apoE3 was obtained by RT-PCR from normal human liver tissue. Site-directed mutagenesis was used to obtain the cDNAs encoding apoE2 and apoE4 isoforms. The 3 cDNAs were subcloned into vector pGEM-3Z and verified by DNA sequencing. The expression recombinant which can express the target protein as a (His) 6-tagged fusion was constructed by subcloning apoE cDNA into vector pT7-PL. The purified proteins were gained by Ni-NTA column. The SDS-PAGE results revealed the 6 His fusion proteins (apoE2...

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Cloning and Expression of Apolipoprotein E3 and Its Variant apoE2 and apoE4

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