The effect of carbon sources, nitrogen sources, and other cultural characteristics for the heterotrophic Chlorococcus sp. isolated in this study were researched. The amino acid contents and compositions of Chlorococcus sp. cultured with different nitrogen sources and concentrations were analysis. The results indicated that the optimal carbon sources and nitrogen sources were glucose and urea, the optimal temperature is about 30 ℃ and pH is 7.0-8. 0 growing under heterotrophic condition. The doubling time is about 5 h when it is cultured under ...