Sticker model was improved, calculation methods of cut set using biochemistry experiment was found, and DNA (deoxyribonucleic acid) algorithm of minimal spanning tree problem based on the methods was put forward. Separation experiment was first extended, which contained separation experiment based on separation board and separation experiment based on electrophoresis technique. The DNA algorithm of minimal spanning tree problem first breaks calculation model of DNA computing-to form minimal spanning tree gradually by means of solving minimal ...