期刊论文

中文

华中农业大学学报

1000-2421

2013

32

5

106-111

10.3969/j.issn.1000-2421.2013.05.019

中央级公益性科研院所基本科研业务费专项

本校为其他机构

为探讨不同地理群中华鳖的pomc基因的多态性,建立区分中华鳖不同地理群的分子遗传标记,采用PCR-RFLP的方法扩增得到4个不同地理群(太湖鳖群体、沙鳖群体、台湾鳖群体和黄河鳖群体)中华鳖的pomc基因,比较4个地理群中华鳖的pomc基因的同源性,构建遗传进化树,对其亲缘关系进行分析,并用AccⅡ、BscBⅠ、AsuⅠ和TscⅠ4种限制性内切酶对pomc基因进行酶切分析。结果显示:4个不同地理群的中华鳖均能扩增出786 bp的pomc基因。亲缘关系分析表明:在遗传上台湾鳖和沙鳖亲缘关系较近,而太湖鳖与黄河鳖亲缘关系较近。用酶切分析pomc基因扩增产物,结果表明:用AccⅡ内切酶对4种中华鳖pomc基因进行酶切,其中沙鳖切...

To establish a molecular genetic marker for identifying different populations of Pelodoiscus sinensis,pomc gene was amplified by PCR and identified by RFLP using four restriction endonucleases including AccⅡ,BscBⅠ,AsuⅠ and TscⅠ.At the same time homology were compared and phylogenetic trees were constructed.The results showed that a 786 bp fragment was amplified from four P.sinensis populations.The relationship between Taiwan-introduced and Pelodiscus axenaria populations was closer and the relationship between Taihu Lake and Huanghe River p...