期刊论文

中文

中国无机分析化学

2095-1035

2022

12

5

98-104

10.3969/j.issn.2095-1035.2022.05.015

2017AKB073:鄂西民族专项资助项目 2021-005:恩施土家族苗族自治州农业科学院青年科技创新基金项目

本校为第一机构

针对酶解法测定硒代氨基酸操作复杂,时间长,水解不完全且水解产物不稳定等问题,建立一种微波水解-液相色谱-原子荧光光谱仪测定硒蛋白中硒代氨基酸的方法.样品经HCl(6 mol/L)微波水解后,调节pH值至7.5,采用阴离子色谱柱,pH=6.0的(NH4)2 HP04(40 mmol/L)溶液为流动相,流速0.6 mL/min,等度洗脱12 min的条件对硒代胱氨酸(SeCys2)、甲基-硒代半胱氨酸(MeSeCys)和硒代蛋氨酸(SeMet)进行分离,用原子荧光光谱仪测定其含量.微波水解条件采用星点设计-响应面法进行优化,优选最佳水解条件为:称样量20 mg,水解温度150℃,水解时间40 min.SeCys2、MeSeCys和SeMet均在0～100 ng浓度范围内呈线性关系,相关系数均...

Aiming at the complicated operation,time-consuming,incomplete hydrolysis and unstable hydrolysis products of enzymatic hydrolysis,we have established a microwave hydrolysis-liquid chromatography-atomic fluorescence spectrometry method for the determination of selenoamino acids in selenoproteins.After microwave hydrolysis with HCl(6 mol/L),the sample was adjusted to pH value of 7.5,then the selenocystine(SeCys2),Se-methylselenocysteine(MeSeCys)and selenomethionine(SeMet)were separated by anion chromatography with pH=6.0,40 mmol/L(NH4)2HPO4 solut...