期刊论文

中文

淡水渔业

1000-6907

2012

42

4

3-8

10.3969/j.issn.1000-6907.2012.04.001

武汉工业学院研究生科研创新资金（2010cx020）；

本校为第一机构

从100 g左右的健康草鱼(Ctenopharyngodon idellus)肠道中分离出3株疑似芽孢杆菌(Bacillus)菌株(G1、G2、G3),并通过对其进行生理生化特征、16S rDNA全序列和产酶能力的分析,筛选出1株高产酶能力的益生菌株。结果显示:G1、G2和G3菌株与枯草芽孢杆菌在16S rDNA序列相似性≥99%的水平上聚为同一分支,结合形态观察和生理生化特征,最终鉴定G1、G2和G3菌株均为枯草芽孢杆菌(Bacillus subtilis);以水解圈直径/菌落直径比值评定了G1、G2和G3菌株的产纤维素酶、淀粉酶和蛋白酶的能力,其中G1菌株产纤维素酶、淀粉酶和蛋白酶能力(比值分别为2.9、2.2和3.3)均高于G2、G3菌株,具有作为益生菌的潜力。

Three suspected Bacillus strains （G1, G2, G3 ） were isolated from intestine of healthy grass carp （100g）, and one high ectoenzyme-producing bacillus strain was screened by the way of biochemistry characteristic, 16S rDNA se- quences and ectoenzyme-producing analysis. The results showed that G1, G2, G3 and Bacillus subtilis were clustered at similarity levels （ = 99% ） of 16S rDNA sequences. The identification showed that all of G1, G2, G3 were Bacillus subti- l/s by the way of morphology, biochemistry characteristic and 16S rDNA sequences...