期刊论文

中文

淡水渔业

1000-6907

2022

52

3

66-73

10.3969/j.issn.1000-6907.2022.03.009

2021CFB265:湖北省自然科学基金 HBMQ202012:湖北美琪生物科技有限公司课题 TMKJZ1912:福建省特种水产配合饲料重点实验室开放课题

本校为其他机构

为查明湖北石首大垸农场养殖的黑斑蛙(Rana nigromaculata)歪头病病原与致病原理,本研究应用微生物分离技术,从病蛙脑、肝、脾和肾脏组织中分离、纯化病菌,采用形态特征、生理生化鉴定、16S rRNA基因序列分析进行细菌种类鉴定,通过组织病理学方法分析患病蛙病理损伤,及人工回归感染确定其致病性,并利用纸片扩散法进行药物敏感性试验。结果显示:分离菌(BSH3)与米尔伊丽莎白菌(Elizabethkingia miricola)聚为一支,结合该菌的形态学特征和生理生化特征,综合鉴定为米尔伊丽莎白菌。组织病理切片结果表明分离菌(BSH3)会导致蛙脑组织基质水肿,基质中出现空泡和炎性细胞浸润的现象;肝、脾和肾脏组织出现...

The bacteria were isolated and purified from brain,liver,spleen and kidney of diseased frogs by microbial isolation technology to find out the etiology and pathogenesis of Rana nigromaculata crooked head disease cultured in Dahuan farm,Shishou,Hubei Province.The bacteria were identified by morphological characteristics,physiological and biochemical identification and 16S rRNA gene sequence analysis.The pathological damage of diseased frogs was analyzed by histopathological method,and the pathogenicity of isolated bacteria was determined by arti...