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Atmospheric nanoparticles affect vascular function using a 3D human vascularized organotypic chip

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成果类型:
期刊论文
作者:
Li, Yan;Wu, Yang;Liu, Yan;Deng, Qi-Hong;Mak, Michael*;...
通讯作者:
Mak, Michael;Yang, Xu
作者机构:
[Liu, Yan; Wu, Yang; Li, Yan] Wuhan Polytech Univ, Coll Food Sci & Engn, Key Lab Deep Proc Major Grain & Oil, Chinese Minist Educ, Wuhan 430023, Hubei, Peoples R China.
[Li, Yan; Mak, Michael] Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.
[Deng, Qi-Hong] Cent S Univ, XiangYa Sch Publ Hlth, Changsha 410083, Hunan, Peoples R China.
[Yang, Xu] Cent China Normal Univ, Lab Environm Biomed, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
通讯机构:
[Mak, Michael] Y
[Yang, Xu] C
Yale Univ, Sch Engn & Appl Sci, Dept Biomed Engn, New Haven, CT 06520 USA.
Cent China Normal Univ, Lab Environm Biomed, Sch Life Sci, Wuhan 430079, Hubei, Peoples R China.
语种:
英文
关键词:
Blood vessels;Cell adhesion;Cell engineering;Endothelial cells;Mammals;Nanoparticles;Nitric oxide;Proteins;Toxicity;Biological behavior;Cell adhesion molecules;Human blood vessels;Micro-fluidic devices;Nuclear factor kappaB;Pathological response;Physiological response;Vascular endothelial growth factor;Physiological models;calcium;immunoglobulin enhancer binding protein;intercellular adhesion molecule 1;nanoparticle;nitric oxide;protein corona;protein ZO1;vasculotropin A;animal;Bagg albino mouse;biological model;cell culture technique;chemistry;confocal microscopy;human;lab on a chip;male;metabolism;microvasculature;mouse;pathology;tissue engineering;umbilical vein endothelial cell;Animals;Calcium;Cell Culture Techniques;Human Umbilical Vein Endothelial Cells;Humans;Intercellular Adhesion Molecule-1;Lab-On-A-Chip Devices;Male;Mice;Mice, Inbred BALB C;Microscopy, Confocal;Microvessels;Models, Cardiovascular;Nanoparticles;NF-kappa B;Nitric Oxide;Protein Corona;Tissue Engineering;Vascular Endothelial Growth Factor A;Zonula Occludens-1 Protein
期刊:
Nanoscale
ISSN:
2040-3364
年:
2019
卷:
11
期:
33
页码:
15537-15549
基金类别:
This work was supported by the National Natural Science Foundation of China (No. 21607117).
机构署名:
本校为第一机构
院系归属:
食品科学与工程学院
摘要:
Inhaled atmospheric nanoparticles (ANPs) can migrate into human blood vessels. However, the exact pathogenesis has not yet been well elucidated. In this study, a perfusable 3D human microvessel network was constructed in a microfluidic device. This functional 3D micro-tissue partly mimicked the physiological response of human vessels. Intravascular nanoparticles tend to adsorb proteins to form a protein corona. Based on this pathological response, vessel permeability and vasoconstriction resulting from ANP stimulation might be related to vascul...

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