期刊论文

中文

动物学杂志

0250-3263

2006

41

4

22-26

10.3969/j.issn.0250-3263.2006.04.005

国家自然科学基金重点项目（No.30370232,39730090）； 中国科学院重要创新方向资助项目（No.KSCX2-SW-103,KSCX2-1-03）；

本校为其他机构

随机扩增杂交微卫星 （ Random amplified hybridization microsatellites, RAHM）是一种复合RAPD扩增和寡核苷酸扫描的方法。该方法能够从RAPD产物凝胶上获得更多的信息，具有分析方法快速，高敏感性，能检测到高水平的多态性等优点。RAHM方法通过对RAPD扩增的DNA片段进行微卫星杂交来替代限制性内切酶对基因组DNA的消化，有助于揭示微卫星基因组克隆，进行微卫星引物的筛选。本文采用随机扩增杂交微卫星方法来检测小型哺乳动物大仓鼠（Techerskia triton）种群的遗传多态性，结果表明RAHM方法能够检测到大仓鼠种群中较高的多态性以及种群间的差异，这些条带模式可能代表真核基因组中另一种多态...

Random amplified hybridisation microsatellites （RAHM）, a new system which combines RAPD amplification and oligonucleotide screening, can provide more information from analysis on RAPD gels, and it has several advantages including rapid analysis and high sensitivity for detecting high-level variability. RAHM is useful for revealing microsatellite genomic clones and selecting the microsatellite primers by substituting restrictions enzyme digestion of genomic DNA with microsatellite hybridization. Our study based on RAHM analysis revealed that t...