China Ocean Mineral Resources R & D Association [DY135-B2-06] Funding Source: Medline
机构署名：
本校为第一且通讯机构
院系归属：
生命科学与技术学院
摘要：
AIMS: A thermostable endo-mannanase from the fungus Talaromyces cellulolyticus was identified to facilitate manno-oligosaccharide preparation from Konjac (Amorphophallus konjac) flour. METHODS AND RESULTS: A putative endo-1,4-beta-mannanase from the T. cellulolyticus was obtained and efficiently expressed by improving its gene dosage in the genome of the host. After cultivation in a bench-top bioreactor for about 120 h, the protein content and enzyme activity of mannanase increased to 3.4 g l(-1) and 17 500 U ml(-1) respectively. Enzymatic characterization showed that this enzyme has an optima...
