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Development of a novel DNA delivery system based on rice bran polysaccharide-Fe(III) complexes.

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成果类型:
期刊论文
作者:
Liu, Liang*;Ni, Danni;Yan, Yujian;Wu, Shuheng;Chen, Xin;...
通讯作者:
Liu, Liang;Liu, Gang
作者机构:
[Chen, Xin; Ni, Danni; Wu, Shuheng; Liu, Liang; Yan, Yujian; Xiong, Xuemin] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
[Guan, Jintao] Wuhan Polytech Univ, Sch Chem & Environm Engn, Wuhan 430023, Peoples R China.
[Liu, Gang] Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
通讯机构:
[Liu, Liang; Liu, Gang] W
Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Peoples R China.
Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430023, Peoples R China.
语种:
英文
关键词:
Biocompatibility;Gene delivery;Polysaccharide-Fe(III) complex
期刊:
International Journal of Biological Macromolecules
ISSN:
0141-8130
年:
2020
卷:
142
页码:
600-608
基金类别:
National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [21602166, 31771925]; Fundamental Research Funds of Wuhan Polytechnic University [2018Y13]
机构署名:
本校为第一且通讯机构
院系归属:
食品科学与工程学院
生命科学与技术学院
化学与环境工程学院
摘要:
Metal complexes, as a type of potential non-virus gene carriers, have gained much attention due to their properties of high charge density and unique three-dimensional structure. This study investigated the potential of polyethyleneimine (PEI)-modified rice bran polysaccharide-Fe(III) complex (PEI-PI) as a safe gene delivery system and explored the effect of Fe(III) on the efficiency of gene transfection mediated by PEI modified rice bran polysaccharide (PEI-P) and PEI-PI. Gel retardation assay was used to study the DNA binding and protection capability, MTT assay was performed to evaluate the...

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