期刊论文

Xiong, HR;Turunen, O

英文

Applied Microbiology and Biotechnology

0175-7598

2022

106

5-6

2017-2027

10.1007/s00253-022-11823-3

This work was financially supported by the project (no. DY135-B2-07) from the China Ocean Mineral Resources R&D Association, Hubei Provincial Technical Innovation Program (no. 2018ABA093).

本校为第一机构

Abstract: A hyperthermostable xylanase XYN10B from Thermotoga maritima (PDB code 1VBR, GenBank accession number KR078269) was subjected to site-directed and error-prone PCR mutagenesis. From the selected five mutants, the two site-directed mutants (F806H and F806V) showed a 3.3–3.5-fold improved enzyme half-life at 100 °C. The mutant XYNA generated by error-prone PCR showed slightly improved stability at 100 °C and a lower Km. In XYNB and XYNC, the additional mutations over XYNA decreased the thermostability and temperature optimum, while el...