期刊论文

He, D.

中文

中国粮油学报

1003-0174

2014

29

7

92-95

“十二五”国家科技支撑计划

本校为第一且通讯机构

为获得高产花生四烯酸的高山被孢霉突变株,利用~(60)Co-γ射线对实验室保藏的高山被孢霉M12进行辐照诱变育种。在辐照剂量为1、2和3 kGy时,致死率分别为73.6%、80.6%和93.8%。以生物量、含油率和花生四烯酸产量为综合评价指标,经初筛和复筛,选育出了一株M12-2-2突变株。经摇瓶发酵,该突变株的生物量为30.73 g/L,含油率为40.51%,ARA产量为4.75 g/L,其ARA产量比出发株提高了1.65倍。经十代传接培养后,该突变株的遗传性能稳定。

In order to get a Mortierella Alpina saltant with a higher production of arachidonic acid, the Mortierella Alpina M12 preserved by our laboratory has been conducted for study by 60Co-γray. When the irradiation dose is 1, 2 and 3 kGy, the lethality is 73.6%, 80.6% and 93.8% respectively. Biomass, oil content and the production of ARA were considered as the comprehensive evaluation indexes. Through preliminary screening and further screening, we could breed a M12-2-2 saltant. After shaking flask fermentation, its biomass was 30.73 g/L;oil length was 40.51%;ARA content 4.75 g/L, production ...