期刊论文

He, Dongping

中文

中国粮油学报

1003-0174

2014

29

11

65-69

“十二五”国家科技支撑计划（2011BAD02B00）；

本校为第一且通讯机构

为了探索在寇氏隐甲藻(crypthecodinium cohnii)突变株的营养生长和油脂积累过程中对碳源和氮源的需求及其影响.通过对照组和5个不同的补糖试验组进行研究,各组经发酵7d收获,并测定生物量、含油量、DHA含量和DHA产量.结果显示各组在发酵培养24 h时,碳消耗量约为22.5％,氮耗约为50％.随着不同时间的补糖,测定碳耗和氮耗虽有小幅度升降,但在144～ 168 h时碳耗、氮耗和碳/氮比基本稳定,各组间差异较小.研究表明,前72 h是寇氏隐甲藻生长的重要时期,当碳/氮比在15∶1左右时补糖最佳;当氮源耗尽时补糖不仅可以促进油脂的产生,而且对DHA的形成也是有利的.

The demand and influence of carbon sources and nitrogen sources of mutant strains of crypthecodinium cohnii was studied during its vegetative stage and the process of oil accumulation. One control group and five different experimental groups are fermented for seven days. Meanwhile, the biomass, oil content and the content and productivity of DHA were measured in the fermentation cultivation. The research showed that, after 24 h of the fermentation period, the carbon and nitrogen consumption of each group is about 22.5% and 50% respectively. The...