期刊论文

Optimization of the Camellia Seed Polypeptide Preparation Technology by Response Surface Methodology

He, D.

中文

中国粮油学报

1003-0174

2016

31

2

109-115

10.3969/j.issn.1003-0174.2016.02.021

国家粮食公益性行业科研专项

本校为第一且通讯机构

针对油茶籽加工副产物利用率低,油茶籽多肽制备得率和纯度不高等问题,本研究利用中性蛋白酶对油茶籽蛋白进行水解,研究了酶解pH、酶解温度、底物浓度、加酶量和酶解时间5个单因素对油茶籽多肽制备的影响,在单因素试验的基础上,选取了底物浓度、酶解温度和加酶量3个因素,采用综合平衡法,以酶解后油茶籽蛋白的水解度、游离氨基酸浓度和多肽浓度隶属度的综合分为指标,进行三因素二次旋转试验设计,并进行响应面分析,以优化油茶籽多肽的制备条件,优化结果底物浓度3.00%、酶解温度47.0℃、加酶量6 440 u/g底物,在该优化条件下的试验显示,油茶籽蛋白水解度19.25%、游离氨基酸浓度76.60 μmol/mL、油茶籽...

In the paper, adopting neutral protease to hydrolyze camellia seed protein in view of the low utilization rate of the by-products from camellia seed and problems about the low yield and purity of camellia peptides preparation technology. The effects of enzymolysis pH, substrate concentration and enzymolysis temperture and enzyme dosage on the preparation of camellia seed polypeptide were studied respectively. On the basis of single factor experiments, the substrate concentration, enzymolysis temperture and enzyme dosage were selected to conduct...