期刊论文

Effect of Wheat Protein Hydrolysate with Rich Glutamine on Mucosal Barrier of DSS Induced Colitis Mice

Wang, X.

中文

中国粮油学报

1003-0174

2016

31

9

7-12

10.3969/j.issn.1003-0174.2016.09.002

武汉轻工大学研究生教育创新计划（2013cx016）；

本校为第一且通讯机构

探讨了富含谷氨酰胺（Gln）小麦水解蛋白对葡聚糖硫酸钠（DSS）诱导小鼠结肠炎的影响。将24只小鼠分为正常对照组（Ⅰ组）、DSS诱导模型组（Ⅱ组）和营养液组（Ⅲ组）。观察了临床表现及结肠黏膜的病理组织变化,检测了结肠组织的抗氧化活性,荧光定量PCR测定了结肠组织中炎症因子的表达。结果表明,营养液处理后,减轻了由结肠炎造成的结肠黏膜的病理损伤,结肠组织中的IFN-γ和IL-6的表达较模型组低,而IL-10和TGF-β的表达差异不显著,表明富含Gln的小麦水解蛋白可以促进促炎/抑炎因子的相对平衡,从而缓解肠道炎症,是一种潜在的抗氧化和抗炎资源。

In order to explore the influence of wheat protein hydrolysate rich in glutamine (Gln) on dextran sodium sulfate (DSS) induced colitis in mice, using DSS induced mice to establish the colitis model, after the completion of colitis model hydrolyzed wheat protein rich in nutrient solution Gln was irrigated for continuous 7 d, and mice would be put to death.Through observing the clinical manifestations and pathological tissue changes of colonic mucosa, colon tissue myeloperoxidase(MPO), superoxide dismutase(SOD), reduced glutathione(GSH), and malo...