期刊论文

He, D.

中文

中国粮油学报

1003-0174

2017

32

10

100-105

10.3969/j.issn.1003-0174.2017.10.018

本校为第一且通讯机构

为了制备生物柴油而获得高产油脂的酵母,分离出1株产油酵母,利用分子生物学技术对其RNA基因内转录区(ITS区)进行了克隆测序,并与GenBank中已有的菌株基因序列进行比对;同时,对该菌株生长发育和油脂合成进行了研究;对生物量、糖氮代谢、油脂含量以及脂肪酸组分进行了检测分析。结果表明:该菌株ITS序列长度为479 bp,与GenBank中近平滑假丝酵母相比较同源率接近100%,形态结果和分子生物学鉴定表明该菌株为近平滑假丝酵母;该菌在0 ~ 12 h期间菌体数量稳定增长,糖氮消耗明显为近平滑假丝酵母的发酵适应期;12 ~48 h,此时菌种快速繁殖,糖氮消耗非常显著,为近平滑假丝酵母的对数期;48 ~ 120 h,菌体数量保...

In order to gain the yeast with high oil yield for preparing biodiesel and isolating one yeast strain, cloning and sequencing of its internal transcribed region (ITS region) of RNA gene was performed by molecular biology technique. Also it is compared with the existing gene sequences in GenBank. At the same time, the growth and development of this strain were studied. The biomass, carbohydrate and nitrogen metabolism, lipid content and fatty acid composition were also test and analyzed in our study. Results showed that firstly the strain ITS se...