期刊论文

中文

中国农业科学

0578-1752

2004

37

8

1119-1122

10.3321/j.issn:0578-1752.2004.08.006

国家自然科学基金，the Foundation for Doctorate Research of Ministry of Education, China

本校为第一机构

应用透射电子显微镜观察水稻淀粉胚乳细胞核在发育期间的形态变化.结果表明,伴随淀粉胚乳发育进程,其细胞核呈现染色质凝聚这一程序性细胞死亡(PcD)的典型特征.水稻胚乳细胞还出现核变形、核膜破裂、核基质进入胞质乃至核降解形成核残体的现象,细胞核从变形到解体是以一种有序的方式进行,证实水稻淀粉胚乳的细胞死亡是程序性细胞死亡.EVANS BLUE染色结果表明,水稻淀粉胚乳细胞死亡顺序是由胚乳中部向四周扩展.随发育进程,水稻胚乳中的超氧物歧化酶(SOD)和过氧化氢酶(CAT)活性持续下降.对水稻淀粉胚乳中的DNA进行凝胶电泳分析,不呈现典型的DNA梯状条带.结果表明,水稻淀粉胚乳细胞的程序性死亡是一...

Morphological variations of the nucleus in starchy endosperm cell were observed by the electron-transmisson microscope during endosperm development in rice. Along with the development of the starchy endosperm,the nuclei of the cells showed chromatin condensation,the typical feature of programmed cell death(PCD). The nuclei also showed nucleus deformation,disruption of nuclear envelope,nucleoplasm leaking into the cytoplasm and nucleus disintegration resulting in nuclear residue formation. From the nucleus deformation to the nucleus disintegration,the morphological changes of the nucleus were o...