期刊论文

中文

中国农业科学

0578-1752

2008

41

7

2149-2153

国家自然科学基金面上项目（30500362）； 湖北省自然科学基金项目（2005ABA091）； 武汉市青年科技晨光计划项目（20055003059-46）；

本校为第一机构

[目的]研究免疫应激对断奶仔猪脾脏、胸腺和外周血白细胞过氧化物酶体增殖物活化受体γ(PPARγ)蛋白表达水平的影响.[方法]选取12头健康断奶仔猪,分成2组,每组6个重复.试验组注射100μg-kg~(-1) BW 的脂多糖(LPS)建立免疫应激模型,对照组注射等量生理盐水.注射后3 h,采血分离血浆和白细胞待测,然后立即屠宰仔猪,取脾脏和胸腺,通过Western blot测定脾脏、胸腺和白细胞中PPAR γ蛋白表达水平.[结果]LPS刺激仔猪,导致血浆白细胞介素-6、肿瘤坏死因子-α、皮质醇和前列腺素E2含量显著升高(P

[Objective] An experiment was conducted to investigate the effect of immunological stress on peroxisome proliferator-activated receptor y （PPARγ） protein expression in spleens, thymus and peripheral leucocytes of weaned pigs. [Method] Pigs were allocated to two treatment groups （6 replicates/treatment and 1 pig/replicate）. The pigs in treatment group were injected with 100 μg·kg^-1 BW lipopolysaccharide （LPS）, whereas in control group were injected with an equivalent amount of sterile saline. Blood samples were collected at 3 h post-ch...