版权说明 操作指南
首页 > 成果 > 详情

Microcalorimetric investigation of the ATPase activity and the refolding activity of GroEL system

认领
导出
Link by 万方学术期刊
反馈
分享
QQ微信 微博
成果类型:
期刊论文
作者:
Chen, Daixiong;Zhang, Ke;Li, Jiaokun;Zeng, Chi;Huang, Qiuxia;...
通讯作者:
Zhu, Jianyu;Gan, Min
作者机构:
[Zhang, Ke; Li, Jiaokun; Zhu, Jianyu; Gan, Min; Chen, Daixiong; Nie, Yunzhong; Huang, Qiuxia] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China.
[Chen, Daixiong; Zhu, Jianyu] Hunan Res Inst Nonferrous Met, Changsha 410126, Hunan, Peoples R China.
[Zeng, Chi] Wuhan Polytech Univ, Sch Biol & Pharmaceut Engn, Wuhan 430023, Hubei, Peoples R China.
通讯机构:
[Zhu, JY; Gan, M] C
[Zhu, Jianyu] H
Cent S Univ, Sch Minerals Proc & Bioengn, Changsha 410083, Hunan, Peoples R China.
Hunan Res Inst Nonferrous Met, Changsha 410126, Hunan, Peoples R China.
语种:
英文
关键词:
Calorimetric;GroEL;Molecular chaperones;ATP hydrolysis;Refolding
期刊:
Journal of Thermal Analysis and Calorimetry
ISSN:
1388-6150
年:
2019
卷:
135
期:
4
页码:
2411-2418
基金类别:
Acknowledgements This research was supported by the National Natural Science Foundation of China (41773089), postdoctoral foundation for MG from Chinese PD science foundation (2017M610506), PD research funding plan in Hunan and central South University (185690), the Shanghai Tongji Gao Tingyao Environment Protection Science and Technology Development Foundation, Opening Foundation of the Chinese National Engineering Research Center for Control and Treatment of Heavy Metal Pollution (No. 2015CNERC-CTHMP).
机构署名:
本校为其他机构
院系归属:
生命科学与技术学院
摘要:
GroEL, a member of the Hsp60 class molecular chaperones, has been extensively studied by general molecular biological method not only on its ATPase activities but also on refolding mechanism. Here, a C80 isothermal calorimeter was applied to understand the molecular mechanism of GroEL-assisted refolding. The power–time curves were obtained from different reaction systems, and the integral heat was calculated and analyzed. The ATPase activity of HSP60 was proven to be very stable at high temperature and had a high temperature dependence. In ATP...

反馈

验证码:
看不清楚,换一个
确定
取消

成果认领

标题:
用户 作者 通讯作者
请选择
请选择
确定
取消

提示

该栏目需要登录且有访问权限才可以访问

如果您有访问权限,请直接 登录访问

如果您没有访问权限,请联系管理员申请开通

管理员联系邮箱:yun@hnwdkj.com