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Phylogenomic analysis of Balantidium ctenopharyngodoni (Ciliophora, Litostomatea) based on single-cell transcriptome sequencing

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成果类型:
期刊论文
作者:
Sun, Zongyi;Jiang, Chuanqi;Feng, Jinmei;Yang, Wentao;Li, Ming*;...
通讯作者:
Li, Ming;Miao, Wei
作者机构:
[Li, Ming; Sun, Zongyi; Li, M; Miao, W] Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
[Li, Ming; Yang, Wentao; Li, M; Miao, W; Jiang, Chuanqi; Miao, Wei] Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.
[Feng, Jinmei] Jianghan Univ, Sch Med, Dept Pathogen Biol, Wuhan 430056, Hubei, Peoples R China.
通讯机构:
[Li, M; Miao, W] W
[Li, M; Miao, W] C
Wuhan Polytech Univ, Hubei Key Lab Anim Nutr & Feed Sci, Wuhan 430023, Hubei, Peoples R China.
Chinese Acad Sci, Inst Hydrobiol, 7 Donghu South Rd, Wuhan 430072, Hubei, Peoples R China.
语种:
英文
关键词:
protozoal DNA;ribosome DNA;algorithm;animal;balantidiasis;Balantidium;Bayes theorem;carp;chemistry;China;classification;fish disease;fishery;gene expression profiling;genetics;Markov chain;Monte Carlo method;nucleotide sequence;parasitology;phylogeny;procedures;sequence alignment;sequence analysis;statistical model;veterinary;Algorithms;Animals;Balantidiasis;Balantidium;Base Sequence;Bayes Theorem;Carps;China;DNA, Protozoan;DNA, Ribosomal;Fish Diseases;Fisheries;Gene Expression Profiling;Likelihood Functions;Markov Chains;Monte Carlo Method;Phylogeny;Sequence Alignment;Sequence Analysis, RNA
期刊:
PARASITE
ISSN:
1252-607X
年:
2017
卷:
24
页码:
43
基金类别:
Acknowledgements. This work was supported by the Natural Science Foundation of China (Project Nos. 31372168, 31471978, and 31501854) to WM, ML and JF.
机构署名:
本校为第一且通讯机构
院系归属:
动物科学与营养工程学院
摘要:
In this paper, we present transcriptome data for Balantidium ctenopharyngodoni Chen, 1955 collected from the hindgut of grass carp (Ctenopharyngodon idella). We evaluated sequence quality and de novo assembled a preliminary transcriptome, including 43.3 megabits and 119,141 transcripts. Then we obtained a final transcriptome, including 17.7 megabits and 35,560 transcripts, by removing contaminative and redundant sequences. Phylogenomic analysis based on a supermatrix with 132 genes comprising 53,873 amino acid residues and phylogenetic analysis based on SSU rDNA of 27 species were carried out ...

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